WebNov 1, 2024 · The yeast cells that lost the plasmids were grown on a solid YPD medium, yielding strain BY4742A. Strain BY4742 was cultivated with strain BY4741 in 1 mL of YPD medium at 30 °C for 1.5 h, with an initial optical density at 600 nm (OD 600) of 0.1 for each haploid cell type. After cultivation, yeast cells were harvested, washed, and resuspended ... WebAug 15, 2024 · The artificial pathways were integrated into yeast genome, and the recombinant S. cerevisiae yZCH21 was constructed as follows: a 500 bp fragment (HO-L) from the HO locus of BY4742 genomic DNA was amplified by PCR, and then HO-L and the first terminator of cassettes were assembled in order by OE-PCR as up homologous …
BY4741 SGD
WebSep 21, 2016 · YXM10: strain with cytoplasm engineering; BY4742-M-04, strain with mitochondria engineering; BY4742-MC-01, haploid strain with a mixed cytosolic-mitochondrial strategy; BY4742-C-05, haploid... Webthe BY4742 strains, His+ frequencies peaked after two or three subcultures and then noticeably declined (Fig. 1C, left panel). For the GRF167 strains, the highest His+ fre-quencies were obtained after four to five subcultures, but they then decreased only modestly (Fig. 1C, right panel). The median peak His+ frequencies for the BY4742-de- first wedding anniversary flowers
Nutritional requirements of the BY series of - Oxford Academic
WebMar 5, 2024 · Yeast whole genome sequencing (WGS) lacks end-to-end workflows that identify genetic engineering. ... CEN.PK113-7D, W303-α, BY4741, BY4742, and K. … http://qikan.cqvip.com/Qikan/Article/Detail?id=48005655 WebNov 1, 2024 · The yeast cells that lost the plasmids were grown on a solid YPD medium, yielding strain BY4742A. Strain BY4742 was cultivated with strain BY4741 in 1 mL of … camping connect in van wifi