Cifwt

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Cifwt

WebANNUAL MEETING: Please join us for our 93rd Annual Meeting & Member Appreciation … Web(B) Anti-biotin western blot showing titration of the indicated amount of Cifwt or CifC/A with 200 nM SCFFbw7ΔD, prior to 10-min long polyubiquitylation reactions for a biotin-labeled cyclin E phosphopeptide. In all these reactions, the indicated version of Cif was preincubated with NEDD8-modified SCFFbw7ΔD for 1 h prior to reaction initiation.

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WebCif decreases neddylated SCF-mediated substrate polyubiquitylation in vitro. (A) Anti-biotin western blot showing time courses of polyubiquitylation of a biotin-labeled cyclin E p

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