WebJan 5, 2024 · SummaryAutomatic TranslationJanuary 5th, 2024. Here, a method is described for making plant tissues transparent while maintaining the stability of fluorescent proteins. This technique facilitates deep imaging of … WebAmong them, a protocol called ClearSee efficiently clears tissues and diminishes chlorophyll autofluorescence while maintaining fluorescent proteins - thereby allowing analysis of …

ClearSeeAlpha: Advanced Optical Clearing for Whole …

WebCentOS Linux 6.4 64-bit x 86 (English only) Minimum Specifications for Allot ClearSee Cluster Nodes. Cluster Data Warehouse Hardware. IBM x3650 M4 2U server. 2 x Intel Xeon with 8 cores running at 2.0Ghz. 128 GB RAM. 7.3 TB of server storage via 12 x 600 GB disks. Dual AC/DC 750W power supply. WebDriving Directions to Tulsa, OK including road conditions, live traffic updates, and reviews of local businesses along the way. downloadstring 超时

Improved clearing method contributes to deep imaging of plant

WebThe ClearSee protocol was performed as previously described (Kurihara et al., 2015) with several modifications. Leaf blades were cut into 5-mm-long segments and fixed in 4% (w/v) formaldehyde solution (freshly prepared from paraformaldehyde and containing 0.1% Silwet L-77) for 1.5 to 3 hr under vacuum at room temperature. For the imaging of ... WebClearSee alpha provided a reduced pink coloration (Fig. 1g, h, k–r). However, the clearing duration and eciency of these methods were dierent. FAST9 removed PAs within 3–5 days of incubation (Fig. 1g, h). For CHAPS clear and ClearSee alpha, it took about 15 days to remove most of the PAs (Fig. 1k-r). However, we did observe pink staining WebJan 20, 2024 · Here, we describe a ClearSee-based clearing protocol which is suitable for obtaining 3D images of Arabidopsis thaliana embryos. The method consists of embryo … clausen\\u0027s racing supply