NettetIn this chapter, we describe a practical method for detecting and quantifying neurodegeneration in adult and aging mouse brains based on protocols developed in our laboratory over the last decade. NettetEmbed the tissue in paraffin at 58 °C. Tissues can be embedded into paraffin using specialized automated tissue processing systems. Cut 5 - 15 µm thick tissue sections using a rotary microtome. Float the sections in a 56 °C water bath. Mount the sections onto either ProbeOn, or ProbeOn Plus or gelatin-coated histological slides.
Combining Multiplex Fluorescence in situ Hybridization with
Nettet23. jul. 2024 · TBA-Dehydrating Paraffin Embedding. For mouse brains, the brain was first removed from the skull and transferred to neutral buffered PFA (4% w/v in 10 mM … Nettet5. sep. 2024 · As the specimens are dehydrated completely and embedded in wax, the paraffin blocks can be stored indefinitely. In addition, paraffin sectioning produces thin sections that improve biological probe access in further experiments and reduce cell layer overlay in the Z direction. home health paso robles
Paraffin embedding for mouse tissue - protocols.io
NettetIn this chapter, we describe a practical method for detecting and quantifying neurodegeneration in adult and aging mouse brains based on protocols developed in our laboratory over the last decade. NettetEmbedding is important in preserving tissue morphology and giving the tissue support during sectioning. Some epitopes may not survive harsh fixation or embedding. The tissue is typically cut into thin sections (5-10 µm) or smaller pieces (for whole mount studies) to facilitate further study. Paraffin-embedded tissue. Nettet8. jul. 2024 · HistoCore Arcadia H - Heated Paraffin Embedding Station: Leica: 14039357258: Tissue-Tek® Base Molds for Embedding Rings: EMS: 62528-38: HistoCore Arcadia C - Cold Plate: ... The following protocol is optimized for the mouse brain. Place the fixed brain in a large size plastic paraffin cassette (FisherScientific, … home health patient population